(1) The structure of colloidal ore gold
Colloidal Gold, also known by the name gold sol, is the suspension of particles made from gold after the original salt has been reduced. The essential gold core of colloidal gold (atomical silver Au) is surrounded by a double layer of ions. For the maintenance of the suspension of colloidal silver between sols and days, the layer with negative ions (AuC122-) is connected to the core. Colloid gold particles’ primary gold core isn’t ideal for spherical symmetry. Although the colloidal feld of smaller particles is spherical (generally referring to particles larger than 30 nm), those that are greater in weight are almost all elliptical. A electron microscope allows you to observe colloidal-gold’s particle morphology.
(2) Some characteristics of colloidal Gold
1. Colloidal nature. Colloid gold particles typically measure between 100 and 1100 nm in size. These tiny gold particles can be suspended in the liquid in an even distribution to form a colloidal solution. Colloid gold therefore has different properties, particularly in terms of sensitivity to electrodelytes. In order to break the solid state, the electrolyte could destroy the permanent hydrohydration layer on the colloidal golden particles. A few macromolecular molecules, including proteins, can protect and improve the stability of colloidal golden.
It is an often used labeling technology. It’s a brand new immunolabeling technique that utilizes colloidal gold for tracer markers of antigens. It offers many advantages. In biological studies, this label has been extensively used over recent years. The label is used by almost every immunoblotting technique. This label is also useful in electron microscopy and flow cytometry as well.
Chloroauric acid (HAuCl4) is used to create colloidal gold. When reducing agents are applied, such as ascorbic and sodium citrate, white phosphorous, tannic acids, and so on, the resulting particles can be modified into gold particles. It then becomes a stable state of static electricity. The stable state of colloidal colloidal is formed by a negatively-charged hydrophobic glue. Because colloidal golden is positively charged, in an alkaline environment it forms strong bonds that can bond to the positively charged group of protein molecules. Since this bond is electrostatic it doesn’t affect the biological attributes of the protein.
A coating procedure that allows proteins to be adsorbed on the colloidal-gold particles’ surface is called Colloidal Gold Labeling. Adsorption can be explained by the existence of a negative charge on the surface colloidal-gold particles. The negatively charged areas form a strong bond to positively charged elements of proteins due electrostatic adsorption. Chloroauric Acid can be used to make colloidal silver particles, which are of different sizes. This cylindrical particle can be used to absorb protein. It is non-covalently compatible with staphylococcal Protein A, immunoglobulins. Because of this, it is a great tool in basic and clinical research.
Common detecting technology
Immunocolloid Gold Staining
Staining cell sections with colloidal silver-labeled antibodies or suspensions of cells can enhance the sensitivity to colloidalgold labeling.
Immunocolloid gold electron microscope staining
The antibodies and anti-antibodies labeled by colloidal silver can be mixed with negatively stained virus samples, or ultrathin tissues sections to make them negatively stained. It can be used in the identification and detection of virus morphology.
Dot immunogold filtration
A microporous membrane, such as a membrane, can be used as a carrier. Once the antigen has been identified on the surface of the layer, the sample should then be added to the seal and washed with colloidal-gold-labeled antibodies to confirm the detection.
Colloidal gold immunochromatography
The membrane is coated with the antibody specific to it in a slime shape. On the binding surface, the colloidal golden labeling reagent or monoclonal antibody is adsorbed. To test the specimen, add it to the bottom of the test strip. With the naked eye, you can observe the color development results on the test-strip. This has resulted in a very user-friendly diagnostic test strip.
The rapid development of standard gold standard technology
Once the antibody has been attached to one particular region of the acid cell membrane, it is then fixed. If one side of the dried acidcellulose is placed in the sample (urine/serum), the sample will travel along the membrane because of capillary movement Move. Once the antibody has been fixed to the membrane, any antigen found in that region will be bound to it. Furthermore, gold particles exhibit high electron density at junctions of gold labeled proteins. This was visible by the naked eye when red spots appeared as a result of the accumulation of these markers at the appropriate ligands. This principle underlies the rapid-gold label detection technique. If the sensitiveness and specificity, as well the use of colloidal and monoclonal or polyclonal antibody, antigens, protein-chimeras, and antigens can be reached the highest standards, then the production of fast and accurate reagents will not be hampered. This is gold. It’s the best standard for standard reagent quality.
TRUNNANO (Luoyang Trunnano Tech Co. Ltd.), is a Colloidal golden manufacturer. They have over 12 years of combined experience in chemical products development and research. For high quality Colloidal golden, contact us to send us an enquiry.